Abstract: 【Objectives】 To understand the status quo of terrestrial vertebrate resources in Qinling National Botanical Garden and to get better acknowledgment and protection of the species resources and biodiversity in this area. 【Methods】 Based on the field investigation during 2019-2022 and combined with the existing monitoring data and literature records. The list of terrestrial vertebrates in the region was collected, updated, and analyzed, including classifications, protection levels, endangered levels, fauna and distribution types. 【Results】 The results showed that there are 4 classes, 29 orders, 99 families, 208 genera, and 306 species of terrestrial vertebrates, including 2 orders, 7 families, 11 genera, 11 species in amphibia; 2 orders, 9 families, 15 genera, 18 species in reptilia; 18 orders, 60 families, 143 genera, 234 species in ave; 7 orders, 23 families, 39 genera, 43 species in mammalia. Compared with the 142 species recorded in the Scientific Investigation Report of Qinling Botanical Garden, 164 species were added. Among these, 31 species were endemic to China, 69 species were listed as national and provincial class key protected wildlife, and 25 species were threatened species. In terms of zoogeographical division, the Oriental species dominated (45.42%) with the existence of Palaearctic species (31.70%) and widespread species (22.87%). From the perspective of distribution types, the animals belonged to 13 distribution types, among which the Oriental pattern, Palaearctic pattern, Himalayan-Hengduan Mountain pattern species were dominant, and other distribution patterns also occupied a certain proportion. By analyzing the G-F index of each class, we found that the G index, F index, and G-F index of birds were the highest, followed by mammals and amphibians. 【Conclusions】 Qinling National Botanical Garden was located in the confluence area of Oriental and Palaearctic species. It had high terrestrial vertebrate richness and many key protected and endemic species. The number of species showed a significant growth trend, indicating that there was a possibility of underestimation of species diversity. At the level of family and genus diversity, birds were relatively abundant, mammals were second, and amphibians were lower. Therefore, it could be suggested to further increase the scope and frequency of investigation and use a variety of techniques to carry out the species background investigation.