Abstract: To better qualify children's perception of nature, this paper purposed sense of gaining nature theory based on the theories of connectedness to nature and sense of gain. Sense of is the subjective feeling of human beings based on the actual materials and spiritual acquisition. This study collected 410 questionnaires finished by children and adopted the scale development method of exploratory factor analysis and confirmatory factor analysis to develop a “Sense of Gaining Nature Scale for Children (SGNS-C)” with 17 items. It was found that urban children’s sense of gaining nature consists of two dimensions: the sense of gaining knowledge and skills and the sense of direct actual gaining. Further research found that children's sense of gaining nature and its two dimensions had a significant positive impact on their household waste sorting behavior.